書誌事項
- タイトル別名
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- TRPV4 protects OVA-induced food allergy in mice via maintaining colonic epithelial barrier functions
抄録
<p>The prevalence of food allergy has increased worldwide but the pathogenesis remains undefined and effective treatments has not been established. Transient receptor potential vanilloid 4 (TRPV4), a mechanosensitive nonselective cation channel, is mainly expressed in epithelium of various organs. The present study investigated the role of TRPV4 in the pathogenesis of ovalbumin (OVA)-induced food allergy in mice. TRPV4 was mostly expressed in colonic epithelium. Repeated oral OVA challenge after sensitization induced food allergy, characterized by systemic allergic symptoms, diarrhea, upregulation of Th2-cytokines such as IL-4, IL-5, IL-13, and increase in serum OVA-specific antibodies, but all these responses were significantly augmented in TRPV4-deficient (TRPV4KO) mice compared with wild-type (WT) mice. Infiltration of CD11c-, CD117-, CD4-, and CD170-positive cells in the colon with OVA-induced food allergy was also enhanced in TRPV4KO mice compared with WT mice. Intestinal permeability determined by the FITC-dextran method was significantly increased in TRPV4KO mice compared with WT mice in normal and OVA-induced food allergy. Furthermore, the expression of adherence junction protein E-cadherin, tight junction protein claudin-3 and occludin in the colon was significantly lower in TRPV4KO mice than WT mice in normal and food allergy. These results suggest that epithelial TRPV4 protects OVA-induced food allergy. This response may be accounted for by suppressing the penetration of allergens via maintaining epithelial barrier functions.</p>
収録刊行物
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- 日本薬理学会年会要旨集
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日本薬理学会年会要旨集 97 (0), 1-B-SS05-2-, 2023
公益社団法人 日本薬理学会
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詳細情報 詳細情報について
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- CRID
- 1390298742738629248
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- ISSN
- 24354953
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- Crossref
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- 抄録ライセンスフラグ
- 使用不可