マウスにおけるポドサイトとボウマン嚢上皮細胞の加齢性変化と性差の検討

<p>The dialysis population has expanded rapidly with the aging of the worldwide population. Although chronic kidney disease (CKD) is more common in women than men, the male dialysis population is larger than female. In healthy aging kidney, density of podocyte and parietal epithelial cell (PEC) decreases and expressions of EMT, pericyte marker, and fibrotic thickening in PEC increase. PEC appears to be a source of new podocytes after podocyte injury. However, the relationship between sex differences and changes in PEC in aging has not been elucidated. We examined changes in glomeruli histologically using 4, 12, 18, 24, 27 months old male and female mice, assumed 26, 58, 64, 70, 79 years old in human age. The podocyte density decreased from 4 months (mo) to 12mo in both male and female mice. After 12mo, podocyte density in only female mice decreased until 24mo. The glomerular tuft area in male and female increased from 4mo to 12mo and from 4mo to 24mo, respectively. Albuminuria in male aging mice did not change, while that in female at 18mo and 27mo was higher than that at 4mo. CD44, a marker of activated PEC increased after podocyte injury and aging. In male, the CD44-positive PECs of glomeruli in outer cortical (OC) increased from 12mo and those in juxta-medullary (JM) increased from 4mo. In female, those in OC and JM increased from 24mo and 18mo, respectively. Nephrosclerosis appeared from 24om in male and 27mo in female. These results suggested that renal injury in aging occurs earlier in male mice and hyperfiltration continues older in female mice.</p>