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- Takeo Saneyoshi
- Dept. Pham. Kyoto Univ. Grad. Sch. Med.
Bibliographic Information
- Other Title
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- 液―液相分離が維持するCaMKIIの分子活性
Description
<p>Liquid-liquid phase separation (LLPS) is a phenomenon in which different proteins spontaneously assemble within a cellular compartment that recognized as a membraneless organelle. LLPS plays important roles in neurons, particularly in the structural organization of postsynaptic densities.</p><p>Calmodulin-dependent protein kinase II (CaMKII) is a major synaptic protein kinase and a key regulator of synaptic plasticity. CaMKII forms calcium-gated condensates with the glutamate receptor subunit GluN2B via LLPS. Since the interaction with GluN2B maintains CaMKII in an active conformation, we hypothesize that the LLPS of CaMKII serves as a molecular machine to maintain biochemical activity as a memory. Given that a protein in a liquid condensate can be replaced by an external protein, a protein cannot persist in the condensate for long time, but the condensate itself can persist for a long time. This unique property reasonably explains how the condensate of CaMKII can be a memory unit that lasts longer than the lifetime of the protein. </p><p>In this symposium, I will present our recent progress on how CaMKII maintains its activity at the synapse using the in vitro reconstituted LLPS assay and nanobody-assisted synaptic translocation of CaMKII into the synapse combined with the FRET sensor of CaMKII.</p>
Journal
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- Proceedings for Annual Meeting of The Japanese Pharmacological Society
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Proceedings for Annual Meeting of The Japanese Pharmacological Society 97 (0), 2-B-S30-1-, 2023
Japanese Pharmacological Society
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Details 詳細情報について
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- CRID
- 1390298742739026688
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- ISSN
- 24354953
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- Text Lang
- ja
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- Data Source
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- JaLC
- Crossref
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- Abstract License Flag
- Disallowed