Basic study and examination of atypical cell samples in the body fluid mode of the XR-Series Automated Hematology Analyzer

<p>Recently, hematology analyzers have been used for puncture fluid tests because they are easy to use and generate results quickly and consistently. In this study, we evaluated the basic performance, atypical cell detectability, and the relationship between three-dimensional scattergrams which is new function of the XR-Series and cell morphology confirmed visually in the body fluid mode of the XR-Series, an automated hematology analyzer newly developed by Sysmex. Performance sensitivity, dilution linearity, and correlation with visual microscopy and the Sysmex XN-Series showed good results, suggesting that the XR-Series has sufficient capability to perform body fluid tests. In the evaluation of atypical cell detectability using the measured values, HF-BF# (a research parameter) was the most significant in the monomeric category. Significant items were obtained for the detection of atypical cells in the items defining the features of two-dimensional scattergrams, and higher detectability was confirmed by combining them with the measured values (HF-BF# and LY-BF%: research parameters). In the study of three-dimensional scattergrams, as a feature of WDF (EXT) scattergrams of atypical cell samples, the presumed atypical cell population was plotted in the medium to high FSC (forward scattered light intensity), SSC (side scattered light intensity), and SFL (side fluorescence intensity) regions. In terms of SFL, which reflects the amount of nucleic acid, atypical cells with increased nuclear chromatin showed medium to high levels, whereas mesothelial cells and histiocytes showed low levels, showing significant differences. In the future, we expect to investigate the atypical cell detection function by accumulating research findings on scattergram features and three-dimensional scattergram findings.</p>