Contribution of monocyte and macrophage extracellular traps to neutrophilic airway inflammation in severe asthma
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- Quoc Quang Luu
- Department of Allergy and Clinical Immunology, Ajou University School of Medicine Department of Biomedical Sciences, Ajou University School of Medicine
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- Cao Thi Bich Tra
- Department of Allergy and Clinical Immunology, Ajou University School of Medicine Department of Biomedical Sciences, Ajou University School of Medicine
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- Moon Ji-Young
- Department of Allergy and Clinical Immunology, Ajou University School of Medicine
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- Jang Jae-Hyuk
- Department of Allergy and Clinical Immunology, Ajou University School of Medicine
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- Shin Yoo Seob
- Department of Allergy and Clinical Immunology, Ajou University School of Medicine
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- Choi Youngwoo
- Department of Allergy and Clinical Immunology, Ajou University School of Medicine
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- Ryu Min Sook
- Department of Allergy and Clinical Immunology, Ajou University School of Medicine
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- Park Hae-Sim
- Department of Allergy and Clinical Immunology, Ajou University School of Medicine Department of Biomedical Sciences, Ajou University School of Medicine
抄録
<p>Background: Increased blood/sputum neutrophil counts are related to poor clinical outcomes of severe asthma (SA), where we hypothesized that classical monocytes (CMs)/CM-derived macrophages (Mφ) are involved. We aimed to elucidate the mechanisms of how CMs/Mφ induce the activation of neutrophils/innate lymphoid cells (ILCs) in SA.</p><p>Methods: Serum levels of monocyte chemoattractant protein-1 (MCP-1) and soluble suppression of tumorigenicity 2 (sST2) were measured from 39 patients with SA and 98 those with nonsevere asthma (NSA). CMs/Mφ were isolated from patients with SA (n = 19) and those with NSA (n = 18) and treated with LPS/interferon-gamma. Monocyte/M1Mφ extracellular traps (MoETs/M1ETs) were evaluated by western blotting, immunofluorescence, and PicoGreen assay. The effects of MoETs/M1ETs on neutrophils, airway epithelial cells (AECs), ILC1, and ILC3 were assessed in vitro and in vivo.</p><p>Results: The SA group had significantly higher CM counts with increased migration as well as higher levels of serum MCP-1/sST2 than the NSA group. Moreover, the SA group had significantly greater production of MoETs/M1ETs (from CMs/M1Mφ) than the NSA group. The levels of MoETs/M1ETs were positively correlated with blood neutrophils and serum levels of MCP-1/sST2, but negatively correlated with FEV1%. In vitro/in vivo studies demonstrated that MoETs/M1ETs could activate AECs, neutrophils, ILC1, and ILC3 by increased migration as well as proinflammatory cytokine production.</p><p>Conclusions: CM/Mφ-derived MoETs/M1ETs could contribute to asthma severity by enhancing neutrophilic airway inflammation in SA, where modulating CMs/Mφ may be a potential therapeutic option.</p>
収録刊行物
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- Allergology International
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Allergology International 73 (1), 81-93, 2024
一般社団法人日本アレルギー学会
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詳細情報 詳細情報について
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- CRID
- 1390298930854508416
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- ISSN
- 14401592
- 13238930
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
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- 抄録ライセンスフラグ
- 使用不可