Transcriptomic Comparison of Human Hepatoma Huh-7 Cell Clones with Different Hepatitis C Virus Replication Efficiencies

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  • Inoue Yasushi
    Department of Virology II, National Institute of Infectious Diseases, Japan Pulmonary and Critical Care Unit, Mita Hospital, International University of Health and Welfare, Japan
  • Murakami Kyoko
    Department of Virology II, National Institute of Infectious Diseases, Japan
  • Hmwe Su-Su
    Department of Virology II, National Institute of Infectious Diseases, Japan Department of Infectious Diseases, Internal Medicine, Graduate School of Medicine, University of Tokyo, Japan
  • Aizaki Hideki
    Department of Virology II, National Institute of Infectious Diseases, Japan
  • Suzuki Tetsuro
    Department of Virology II, National Institute of Infectious Diseases, Japan

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<p>Hepatitis C virus (HCV) infection represents a major public health problem throughout the world. The establishment of viral replicons has enhanced our understanding of the mechanism underlying HCV replication. However, the specific virus-host cell interactions involved in HCV RNA replication are not well understood. In the present study, we isolated several human hepatoma Huh-7-derived subclones with a range of HCV RNA replication efficiencies by end-point dilution. Of these, the clones HuhTe4 and HuhTe6 were observed to proliferate at the same rate; however, HuhTe6 supported a significantly greater degree of viral RNA replication. Using cDNA microarray analysis, a total of 36 genes (0.4%) demonstrated variable expression, with a ≧2- fold difference in expression noted between HuhTe4 and HuhTe6. Among genes that are implicated in a variety of functional categories, a subset of these differentially-expressed genes has a role in signal transduction and cell communication, including thioredoxin-interacting protein, Rab6B, sorting nexin 16 and UDPgalactose:ceramide glycosyltransferase. The genes identified in this study should be examined further to determine their roles in HCV RNA replication. The Huh-7 subclones identified in this study provide a tool for identifying novel host factors involved in viral replication.</p>

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