Characterization of class 1 integrons and antimicrobial resistance in CTX-M-3-producing Serratia marcescens isolates from southern Taiwan

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  • Peng Chien-Fang
    Laboratory of Microbiology and Clinical Microbiology, Faculty of Biomedical Laboratory Science, College of Health Sciences, Kaohsiung Medical University, Taiwan Division of Clinical Microbiology, Department of Laboratory Medicine, Kaohsiung Medical University Hospital, Taiwan
  • Lee Mei-Feng
    Department of Laboratory Medicine, Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Taiwan
  • Fu Hsiao-Ting
    Faculty of Biomedical Laboratory Science, College of Health Sciences, Kaohsiung Medical University, Taiwan
  • Chen Yuh-Jyh
    Faculty of Biomedical Laboratory Science, College of Health Sciences, Kaohsiung Medical University, Taiwan
  • Hsu Hui-Jine
    Faculty of Biomedical Laboratory Science, College of Health Sciences, Kaohsiung Medical University, Taiwan

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  • Characterization of Class 1 Integrons and Antimicrobial Resistance in CTX-M-3-Producing <i>Serratia marcescens</i> Isolates from Southern Taiwan

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<p>The objective of this study was to characterize the gene cassettes of class 1 integrons and anti-microbial resistance among CTX-M-3-producing Serratia marcescens isolates from different specimens in southern Taiwan. One hundred and twenty-two isolates (70.5%) of 173 CTX-M-3-producing S. marcescens isolates were positive for class 1 integrons, including 53.3% of blood isolates, 94.1% of urine isolates, and 87.2% of sputum isolates. No class 2 or class 3 integrons were detected in this study. By PCR with primers 5 ́-CS and 3 ́-CS for the amplification of gene cassettes regions, amplicons ranging from 0.7 to 3.0 kb in length were found in 108 (88.5%) of the 122 class 1 integron-containing isolates of CTX-M-3-producing S. marcescens isolates. Ten different types by pattern of amplicons for class 1 integrons were obtained. The Type I amplicon (46.3%) harbors two different class 1 integrons containing the gene cassettes of aadA2 and aadB-catB3, respectively, and was most prevalent in the gene cassette region-positive S. marcescens isolates, followed by the Type II amplicon, which harbors one class 1 integron containing the gene cassette dfrA12-orfF-aadA2 (28.7%). Most of the S. marcescens isolates (66.7%, 8/12) harboring three different class 1 integrons (Type IV amplicon) were found in blood isolates. Class 1 integrons were conjugally transferred to recipients in 92.0% of S. marcescens harboring two different class 1 integrons containing the gene cassettes aadA2 and aadB-catB3, respectively. The transfer rate of class 1 integron carrying dfrA12-orfF-aadA2 was detected in 77.4% of S. marcescens isolates. The results showed that all those isolates with conjugative transfer of integrons carried their class 1 integrons on the conjugative plasmids.</p>

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