Glucose Deficiency Altered Gene Expression and affected Hard Tissue Differentiation in Mouse Osteoblast-like cells

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  • Taguchi Yoichiro
    Faculty of Dentistry, Department of Periodontology, Osaka Dental University
  • Kato Hirohito
    Faculty of Dentistry, Department of Periodontology, Osaka Dental University
  • Li Runbo
    Faculty of Dentistry, Department of Periodontology, Osaka Dental University
  • Nakata Takaya
    Faculty of Dentistry, Department of Periodontology, Osaka Dental University
  • Yamauchi Nobuhiro
    Faculty of Dentistry, Department of Periodontology, Osaka Dental University
  • Azuma Hitoshi
    Faculty of Dentistry, Department of Periodontology, Osaka Dental University
  • Mandai Chiaki
    Faculty of Dentistry, Department of Periodontology, Osaka Dental University
  • Ogata Chizuko
    Faculty of Dentistry, Department of Periodontology, Osaka Dental University
  • Umeda Makoto
    Faculty of Dentistry, Department of Periodontology, Osaka Dental University

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  • マウス骨芽細胞様細胞におけるグルコース欠乏によって発現変動する遺伝子解析と硬組織分化に及ぼす影響

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Abstract

<p>Diabetes mellitus is a major risk factor for periodontal disease, considered as one of systemic disease in the field of periodontal medicine. For mimicking the condition of diabetes mellitus, an over-nourished model, i.e., high glucose environment, is often used. Under-nutrition is often a problem in the aging population, but the effect of under-nutrition on hard tissue regeneration is unknown. In this study, we investigated the effects of glucose deprivation on hard tissue differentiation in mouse osteoblasts.</p><p>Mouse osteoblast-like cells (MC-3T3-E1) were established from mouse femurs in primary culture and immortalized in α-MEM in the presence of a glucose concentration of 100 mg/dL as a physiological control and 0 mg/dL as a model of glucose deprivation. The effects of glucose deprivation on the MC-3T3-E1 osteoblasts gene expression related to glucose metabolism were screened and compared with the presence of alkaline phosphatase and calcium deposition in the extracellular matrix.</p><p>Real-Time PCR Array analysis showed that the expressions of eight genes were decreased by 30% in the glucose-deprived group as compared with the physiological control group at 1 week of culture. The expression of pdk-1 mRNA, a gene involved in osteoblast differentiation and survival, was significantly decreased. BX-912, an inhibitor of PDK-1, decreased the cell proliferative ability and hard tissue differentiation ability in the control group as in the glucose-deprived group.</p><p>These results suggest that hard tissue differentiation in MC-3T3-E1 osteoblasts is mediated by PDK-1, which is involved in glucose metabolism.</p>

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