Platelet aggregation-promoting activity of chitosan sheet with porous structure

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  • 多孔質キトサンシートの血小板凝集促進作用

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<p>In this study, we investigated the platelet aggregation-promoting effects of porous chitosan sheet. Chitosan sponge was manufactured as follows, first, chitosan was dissolved in acetic acid aqueous solution, next frozen to as sherbet ice to obtain homogeneous frozen ice, and the sherbet ice was lyophilized, and then heat-treated. Chitosan sheet (chitosan acetate) was a hemostatic material obtained by pressing the chitosan sponge and finally sterilizing by gamma rays. The chitosan sponge had homogeneous porous structure on the surface and cross section with controlled median pore diameter of 97 μm by adding less than 2% ethanol of the final concentration to chitosan acetate solution, While the chitosan sheet had smaller porous structure with the median diameter of 32 μm. Chitosan sponge and chitosan sheet were absorbed quickly, and chitosan sheet showed a decrease in eluted acetic acid and an increase in breaking strength after water absorption by heat treatment at no less than 85 ℃ for 24h. We evaluated the platelet aggregation-promoting activity of chitosan sheet by measuring platelet factor 4 (PF4) and β-thromboglobulin (β-TG) released from aggregated platelets, after contacting with human platelet rich plasma (PRP). Chitosan sheet significantly released the higher amounts of PF4 and β-TG than gauze, hemostatic bandage of chitosan containing acetic acid and chitosan fiber (p < 0.01). These results suggest that chitosan sheet function as a hemostatic material by promoting platelet aggregation.</p>

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