Photocrosslinkable Artificial Nucleic Acid Probe Based miRNA Biosensor

DOI Web Site 参考文献19件
  • WATANABE Yui
    Department of Frontier Fiber and Technology and Science, Graduate School of Engineering, University of Fukui
  • YAJIMA Shuto
    Department of Frontier Fiber and Technology and Science, Graduate School of Engineering, University of Fukui
  • KODA Maho
    Department of Frontier Fiber and Technology and Science, Graduate School of Engineering, University of Fukui
  • KOTO Ayako
    Nicca Chemical Co., Ltd.
  • TAKAMURA Eiichiro
    Department of Frontier Fiber and Technology and Science, Graduate School of Engineering, University of Fukui
  • SAKAMOTO Hiroaki
    Department of Frontier Fiber and Technology and Science, Graduate School of Engineering, University of Fukui

抄録

<p>Molecular recognition elements like enzymes, antibodies, and nucleic acids, which are involved in specific binding, are important components in biosensing technologies. These biomolecular recognition elements are based on molecular interactions such as hydrogen bonding, van der Waals forces, and hydrophobic interactions. However, these interactions are often affected by the solution environment such as pH, temperature, and salt concentration, which are the rate-limiting factors for biosensing applications. In this study, we focused on molecular recognition using photocrosslinkable artificial nucleic acids. Photocrosslinkable artificial nucleic acids can form covalent bonds with target nucleic acids upon photoirradiation after hybridization. The covalent bonds formed are stronger than those in conventional molecular recognition and are not affected by the solution environment. Herein, we propose a biosensing system that combines molecular recognition by photocrosslinkable artificial nucleic acids, isothermal amplification by hybridization chain reaction, and electrochemical detection of miR-21 as the target molecule, which has recently attracted attention as a cancer biomarker. This technology eliminates non-specific binding and enables biosensing measurements with a suppressed background.</p>

収録刊行物

  • Electrochemistry

    Electrochemistry 92 (2), 022018-022018, 2024-02-29

    公益社団法人　電気化学会

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