Evaluation of Antibiotic Penicillin G Activities Based on Electrochemical Measurement of a Tetrazolium Salt
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- Ikeda Hikaru
- Department of Applied Chemistry, Osaka Metropolitan University
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- Tokonami Akira
- Department of Applied Chemistry, Osaka Metropolitan University
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- Nishii Shigeki
- Department of Applied Chemistry, Osaka Metropolitan University
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- Fujita Masashi
- Department of Applied Chemistry, Osaka Metropolitan University EC Frontier Co.
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- Yamamoto Yojiro
- Department of Applied Chemistry, Osaka Metropolitan University Green Chem. Inc.
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- Sadanaga Yasuhiro
- Department of Applied Chemistry, Osaka Metropolitan University
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- Shiigi Hiroshi
- Department of Applied Chemistry, Osaka Metropolitan University Osaka International Research Center for Infectious Diseases, Osaka Metropolitan University
抄録
<p>This study focused on the electrochemical properties of tetrazolium salts to develop a simple method for evaluating viable bacterial counts, which are indicators of drug susceptibility. Considering that the oxidized form of tetrazolium, which has excellent cell membrane permeability, changes to the insoluble reduced form formazan inside the cell, the number of viable cells was estimated based on the reduction current of the tetrazolium remaining in the bacterial suspension. Dissolved oxygen is an important component of bacterial activity. However, it interferes with the electrochemical response of tetrazolium. We estimated the number of viable bacteria in the suspension based on potential-selective current responses that were not affected by dissolved oxygen. Based on solubility, cell membrane permeability, and characteristic electrochemical properties of the tetrazolium salt 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium, we developed a method for rapidly measuring viable bacteria within one-fifth of the time required by conventional colorimetric methods for drug susceptibility testing.</p>
収録刊行物
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- ＣＨＥＭＩＣＡＬ ＆ ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ ＢＵＬＬＥＴＩＮ
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ＣＨＥＭＩＣＡＬ ＆ ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ ＢＵＬＬＥＴＩＮ 72 (3), 253-257, 2024-03-01
公益社団法人 日本薬学会