Real-time PCR-based Quantitative Detection of <i>Tilletia controversa</i>in the Soil of Hokkaido

DOI Web Site
  • MORI Manami
    Central Agricultural Experiment Station, Hokkaido Research Organization
  • KOMATSU Tsutomu
    Central Agricultural Experiment Station, Hokkaido Research Organization

Bibliographic Information

Other Title
  • 北海道で発生したコムギなまぐさ黒穂病菌のリアルタイムPCRによる土壌からの検出と定量
  • ホッカイドウ デ ハッセイ シタ コムギ ナ マグ サ クロホ ビョウキン ノ リアルタイム PCR ニ ヨル ドジョウ カラ ノ ケンシュツ ト テイリョウ

Search this article

Abstract

<p>Bunt disease of wheat caused by Tilletia controversa poses a serious problem in Hokkaido. The pathogen survives in the soil as teliospores, which serve as a source of infection for several years. This study aimed to develop a method for the quantitative detection of T. controversa from infected soil using real-time PCR with a TaqMan probe. The detection limit of this method was 10 spores/g soil. The number of DNA copies increased as disease severity increased. This method facilitated the quantification of T. controversa inoculum from the infected fields. Thus, disease occurrence may be predicted by testing the soil before sowing.</p>

Journal

Keywords

Details 詳細情報について

Report a problem

Back to top