Objective: The purpose of this study was to investigate the effects of the TOKYO2020 Olympic Games on an interest and participation in sports or exercises in Japanese university students. Methods: We conducted a questionnaire survey on 954 university and master course students about an interest in exercises and current situation about participation in exercises before and after the Olympic Games. Results: Of the 504 students that responded (52.8%), 245 (48.6%) students have been participation in exercises,146 (29.0%) students have been an interest in exercises and without participation in exercises, and 113 (22.4%) students have not been an interest in exercises and participation in exercises before the Olympic Games. Total 127 (25.2%) students changed their interest in exercises and participation in exercises positively, however 376 (74.6%) students did not change after the Olympic Games. There were no students who changed them negatively. Actually, 64 out of 113 (56.6%) students without an interest in exercises before the Olympic Games got an interest in exercises (It has changed positively, however it has not reached to get participation in exercises). These 64 students with having positive changes were more than 49 students without changes. Twenty-eight (10.8%) students who had not exercised just started exercising after the Olympic Games. Screen and inactivity time after the Olympic Games were significantly the shortest in students with participation in exercises twice a week before the Olympic Games (screen: 5.1±2.1h/day, inactivity: 5.1±2.7h/day). These times extended in the order of students with participation in exercises once a week (5.5±2.4h/day, 5.5±2.6h/day), with an interest in exercises (6.1± 2.4h/day, 6.5±3.4h/day) and without an interest in exercises (6.1±2.4h/day, 7.0±3.4h/day). Conclusion: The Olympic Games might be contributed to encourage having an interest in exercises for 56.6% of students without an interest in exercises and to participate in exercises for 10.8% of students without participation in exercises.