Chronic effects of secondary effluent water on <i>Raphidocelis subcapitata</i>
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- MURATA Satomi
- Public Works Research Institute
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- TSUSHIMA Ikuo
- Public Works Research Institute
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- HOUNOKI Daisuke
- Public Works Research Institute Meidenshya Co
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- LEE Suntae
- Public Works Research Institute National Institute of Technology, Hachinohe College
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- YAMASHITA Hiromasa
- Public Works Research Institute
Bibliographic Information
- Other Title
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- ムレミカヅキモを用いた下水処理水の慢性影響評価
Description
<p>In the case of safety assessments for sewage effluents, it is necessary to consider chronic (long-term) effects on aquatic organism in addition to short-term effects. Here, we examined the chronic effects of secondary effluents on Raphidocelis subcapitata by repeated subcultivation. Secondary effluents diluted to 5% and 80% with MillQ water and added C medium were used as tested samples. Precultured algal cells were added in each sample (start concentration: 2x105 cells/ml) and cultured at 25 °C, 1,500 Lux, and 100rpm. After 7 days of incubation, algal cells grown in each sample were replaced into new medium at each treated water-concentration. Specific growth rates of algae cells did not differ among the treated water-concentrations after repeated incubations. On the other hands, repeated cultivations in 5% treated water increased in cell numbers, though no significant change in 80% was observed. Cell sizes in 5% treated water were not significant change after repeated subcultivation, while those in 80% enlarged 2.1-fold at the 8th cultivation compared to 0% control. In the AFLP analysis, the 0% treated water was classified into the same cluster regardless of the number of passages, while the 80% treated water was classified into independent cluster. The concentration of Zn in the treated water used in this study was detected at 47.7 mg/L, which was higher than the other metals. These results may suggest that Zn may one of the factor of cell hypertrophy based on previous reports. *This research was supported by Kurita Water and Environment Foundation.</p>
Journal
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- Annual Meeting of the Japanese Society of Toxicology
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Annual Meeting of the Japanese Society of Toxicology 50.1 (0), P3-259-, 2023
The Japanese Society of Toxicology
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Details 詳細情報について
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- CRID
- 1390299395584753792
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- Text Lang
- ja
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- Data Source
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- JaLC
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- Abstract License Flag
- Disallowed