A Method for Determining the Optimum Temperature for a Polyphenol Separation Process by Reversed Phase Chromatography

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  • NOZAKI Shinya
    Graduate School of Sciences and Technology for Innovation, Yamaguchi University
  • YOSHIMOTO Noriko
    Graduate School of Sciences and Technology for Innovation, Yamaguchi University Biomedical Engineering Center (YUBEC), Yamaguchi University
  • YAMAMOTO Shuichi
    Graduate School of Sciences and Technology for Innovation, Yamaguchi University Biomedical Engineering Center (YUBEC), Yamaguchi University

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  • 逆相クロマトグラフィープロセスにおける最適温度の決定法

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Abstract

<p>A method for determining the optimum temperature for the separation of two polyphenols by polystyrene divinylbenzene resin reversed phase liquid chromatography with the ethanol-water mixture mobile phase was proposed based on the iso-resolution curve concept. The distribution coefficient and the stationary phase diffusion coefficient were formulated as a function of temperature T and ethanol concentration I. From the iso-resolution curve, the productivity P* was calculated as a function of T. It was found that there is an optimum T, where the highest P* can be obtained.</p>

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