Using hemoglobin vesicles to treat operative hemorrhagic shock after pneu- monectomy in dog models: an experimental study
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- NAKANO Kei
- Division of General Thoracic Surgery, Department of Surgery, Tokai University School of Medicine, Isehara, Japan
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- KOHNO Mitsutomo
- Department of General Thoracic Surgery, Saitama Medical Center, Saitama Medical University, Kawagoe, Japan
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- ONOZAWA Hiroto
- Division of General Thoracic Surgery, Department of Surgery, Tokai University School of Medicine, Isehara, Japan
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- HASHIMOTO Ryo
- Division of General Thoracic Surgery, Department of Surgery, Tokai University School of Medicine, Isehara, Japan
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- OIWA Kana
- Division of General Thoracic Surgery, Department of Surgery, Tokai University School of Medicine, Isehara, Japan
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- MASUDA Ryota
- Division of General Thoracic Surgery, Department of Surgery, Tokai University School of Medicine, Isehara, Japan
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- YAMAGUCHI Masatoshi
- Department of General Thoracic Surgery, Saitama Medical Center, Saitama Medical University, Kawagoe, Japan
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- HATO Tai
- Department of General Thoracic Surgery, Saitama Medical Center, Saitama Medical University, Kawagoe, Japan
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- WATANABE Masazumi
- Department of General Thoracic Surgery, Keio University School of Medicine, Tokyo, Japan
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- HORINOUCHI Hirohisa
- Department of General Thoracic Surgery, Saitama City Hospital
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- SAKAI Hiromi
- Department of Chemistry, Nara Medical University, Kashihara, Nara, Japan
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- KOBAYASHI Koichi
- Department of General Thoracic Surgery, Keio University School of Medicine, Tokyo, Japan
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- IWAZAKI Masayuki
- Division of General Thoracic Surgery, Department of Surgery, Tokai University School of Medicine, Isehara, Japan
抄録
<p>Hemoglobin vesicles (HbVs), considered as red blood cell substitutes, are liposomes encapsulating purified hemoglobin, with a phospholipid bilayer membrane (diameter: 250 nm; P50, 28 Torr). In this study, we aimed to investigate HbV function during hemorrhagic shock in lung resection and analyze the details of oxygen delivery. Left pneumonectomy was performed in dogs under mechanical ventilation, followed by rapid exsanguination of approximately 30% of the total circulating blood volume, which led to shock, reducing the mean arterial pressure (MAP) by approximately 60% of baseline. Subsequently, either 5% human serum albumin (HSA) or HbVs suspended in 5% HSA were infused for resuscitation. The MAP only recovered to 75% of baseline after HSA administration, but fully recovered (100%) after HbV administration, with significant differences between the groups (P < 0.005). Oxygen delivery was restored in the HbV group and was significantly higher than that in the HSA group (P < 0.0001). The infusion of HbVs dispersed in a 5% HSA solution compensated for the rapid loss of approximately 30% of the total circulating blood volume in a dog pneumonectomy model, even with impaired lung function. Thus, HbVs can be used for resuscitation from hemorrhagic shock during thoracic surgery.</p>
収録刊行物
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- Biomedical Research
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Biomedical Research 45 (2), 91-101, 2024-03-22
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