Evaluation of Various Cryoablation Protocols in Multiple Cell 1ines using Quantitative Biomarkers of Bioluminescence Imaging: An <i>in vitro</i> Study

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  • Khurelbaatar Bolortuya
    Department of Diagnostic Radiology and Nuclear Medicine, Gunma University Graduate School of Medicine
  • Kartamihardja A. Adhipatria P.
    Department of Diagnostic Radiology and Nuclear Medicine, Gunma University Graduate School of Medicine
  • Miyazaki Masaya
    Department of Radiology, Saitama Medical Center, Saitama Medical University
  • Lamid-Ochir Oyunbold
    Department of Radiology, the First Central Hospital of Mongolia
  • Zhang Xieyi
    Research Institute for Science and Technology, Tokyo University of Science
  • Nakajima Takahito
    Department of Diagnostic and Interventional Radiology, University of Tsukuba Hospital
  • Tsushima Yoshito
    Department of Diagnostic Radiology and Nuclear Medicine, Gunma University Graduate School of Medicine

Abstract

<p>Background & Aims: To investigate whether the optimal treatment protocols of cryoablation differ among various cell lines utilizing bioluminescence imaging (BLI).</p><p>Methods: Eight luciferase-expressing cancer cell lines originated from various organs were used in this study, including lung, renal, liver, breast, colon, prostate, and skin. These cell lines were uniformly seeded in 96-well plates. The cell lines were then divided into four groups: Group 0 (G0), as a control group, and three treatment groups (G1-G3). G1-G3 were subjected to cryoablation at temperatures of －45℃, －60℃, and －80℃, respectively, with varying treatment cycles (single, double, or triple cycle, respectively). Each freeze‒thaw cycle consisted of ten minutes of freezing followed by five minutes of thawing. Finally, the average radiance of the cells was assessed using BLI.</p><p>Results: Cryoablation at “－60℃ and －80℃” showed a significant decrease in average radiance, even after a single cycle of treatment in all cell lines. The average radiance of cells treated with －45℃ was substantially lower than that of untreated cell lines except melanoma.</p><p>Conclusion: We found that －45℃ successfully eradicated all cancer cells across multiple cycles except melanoma. The most effective cryoablation protocol for all cell lines was observed at temperatures of －60℃ or lower.</p>

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