Development of a Dynamic Cell Scaffold for Study of Matrix-driven Epithelial-mesenchymal Transition Progression

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  • YAMAMOTO Shota
    Research Center for Macromolecules and Biomaterials, National Institute for Materials Science
  • NAKANISHI Jun
    Research Center for Macromolecules and Biomaterials, National Institute for Materials Science Department of Nanoscience and Nanoengineering, Graduate School of Advanced Science and Engineering, Waseda University Department of Materials Science and Technology, Graduate School of Advanced Engineering, Tokyo University of Science

Bibliographic Information

Other Title
  • マトリックス誘起上皮間葉転換の研究のための動的細胞足場材料の開発

Abstract

<p>Epithelial-mesenchymal transition (EMT) is a dynamic cellular phenotypic change from an epithelial-like to a mesenchymal-like phenotype, which leads to functional changes in cell migration, invasion and metastasis. Recent research showed EMT is not only induced by soluble factors and gene expression but also by the biochemical and mechanical cues of the extracellular matrix (ECM). However, the effect of quantitative and dynamic changes in the ECM-driven biochemical cues on cellular phenotypes remains unclear. In this study, we developed a photoactivatable substrate that can change the cyclic Arg-Gly-Asp (cRGD) ligand density on a substrate surface based on the photocleavage reaction of the 2-nitrobenzyl compound. We observed the epithelial-to-mesenchymal morphological change in Madin-Darby canine kidney (MDCK) cells upon decreasing the cRGD density on the substrate by photoirradiation. In addition, fluorescence live imaging demonstrated membrane ruffling by the sudden cRGD density decreases, presumably due to the reorganization of the actin cytoskeleton. This photoresponsive cell scaffold is useful for the quantitative and qualitative analyses of the dynamic cellular response to ECM-driven signals, which is expected to help comprehensive understanding of EMT progression.</p>

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 73 (3), 103-109, 2024-03-05

    The Japan Society for Analytical Chemistry

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