Verification of molecular markers of coagulation-fibrinolysis using CN-6500 analyzer

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  • KATOU Yusaku
    Division of Clinical Laboratory, Gifu University Hospital
  • CHOUSA Mitsuhiro
    Department of Pathology, Shubun University Faculty of Nursing
  • KANBE Ayumu
    Division of Clinical Laboratory, Gifu University Hospital
  • UENO Yoshihiko
    Division of Clinical Laboratory, Gifu University Hospital
  • ANDO Honomi
    Division of Clinical Laboratory, Gifu University Hospital
  • SHIRAKAMI Yohei
    Division of Clinical Laboratory, Gifu University Hospital Department of Gastroenterology, Gifu University Graduate School of Medicine
  • KIKUCHI Ryosuke
    Division of Clinical Laboratory, Gifu University Hospital
  • OKURA Hiroyuki
    Division of Clinical Laboratory, Gifu University Hospital Department of Cardiology, Gifu University Graduate School of Medicine

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Other Title
  • 全自動血液凝固測定装置CN-6500を用いた凝固線溶系分子マーカーの基礎性能評価
  • ゼン ジドウ ケツエキ ギョウコ ソクテイ ソウチ CN-6500 オ モチイタ ギョウコ センヨウケイ ブンシ マーカー ノ キソ セイノウ ヒョウカ

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Abstract

<p>The CN-6500 fully automated blood coagulation analyzer (CN-6500; Sysmex Corporation) is an instrument that combines a measurement for coagulation-fibrinolysis molecular markers. In this study, we evaluated the basic performance of the CN-6500 in measuring thrombin-antithrombin III complex (TAT), plasmin-α2 plasmin inhibitor complex (PIC), and tissue plasminogen activator-plasminogen activator inhibitor complex (tPAI-C), which are molecular markers of the coagulation-fibrinolysis. The evaluation included repeatability, reproducibility, linearity, limit of detection, influence of interfering substances, biotin addition test, and correlation evaluation between CN-6500 and HISCL-5000 (Sysmex Corporation). The results of repeatability, reproducibility, linearity and limit of detection were favorable. No influence of hemolytic hemoglobin, bilirubin C and F, or interfering substances was observed. However, the three subject items were affected by biotin as a relative value of −10% or more. The correlation between the instruments (HISCL-5000: x, CN-6500: y) was good for TAT (y = 1.013x − 0.269, r = 0.998), PIC (y = 1.026x − 0.134, r = 0.998) and tPAI-C (y = 0.944x + 0.028, r = 0.999). Although the biotin effect was partially observed, the CN-6500 is considered to be a useful analyser for in-hospital testing of molecular markers of the coagulation-fibrinolysis.</p>

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