<p>Background/Purpose: The significance of oral care has widely spread from dental occupations to other health professionals as society is aging rapidly. Although oral care is an important fundamental nursing activity, there is a lack of systematic and continuing education, on oral care during nurse education, hampering their attitude and skills to oral care. The aim of the study was to investigate factors affecting attitude and motivation, as well as clinical practices regarding oral care performed by nurses working in a tertiary hospital in Japan.</p><p>Materials and Methods: An online cross-sectional survey that investigated three categories on oral care; general attitude, learning and motivation, and clinical practices. The questions were scored on a 5-point scale from Disagree=1 to Agree=5. In each category, total points were calculated and analyzed for the effect of education-related factors and experience.</p><p>Results: Out of 710 nurses eligible for participation in this survey, 248 nurses responded (response rate: 34.9%). All respondents acknowledged the importance of oral care and its impact on general health. The majority (82.3%) acknowledged that proper oral care improves their oral hygiene. On the other hand, 70% of respondents, expressed insufficient time for oral care. Analysis showed that experience with oral care had an effect on attitude and knowledge of oral care compared to educational background.</p><p>Conclusion: This survey illustrated that attitude toward oral care is more dependent on the clinical experiences than the educational background of nurses. (J Osaka Dent Univ 2024; 58: 71-77)</p>