Hypoxic Culture of Osteosarcoma Cells in PDMS Microfluidic Chamber and Plastic Bag
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- Inaam Rafia
- Toyohashi University of Technology
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- Bolontrade Marcela
- Institute for Translational Medicine and Biomedical Engineering (IMTIB) CONICET - University Institute of the Italian Hospital
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- Okamoto Shunya
- Toyohashi University of Technology
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- Shibata Takayuki
- Toyohashi University of Technology
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- Subhra Santra Tuhin
- Indian Institute of Technology
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- Nagai Moeto
- Toyohashi University of Technology
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Description
<p>Drug uptake into tumor cells is critical for therapeutic efficacy, and chemoresistance is a limiting factor in therapeutic success. Chemoresistance and tumor growth are modulated by hypoxia, a state of oxygen deprivation. To understand the role of hypoxia in chemoresistance, simple and user-friendly experimental reproducibility of hypoxic conditions is crucial. Therefore, hypoxic culture in a microfluidic chip needs to be more easily controlled with reduced manual intervention, ensuring the precision required in a microchip device. This paper reports hypoxic and normoxic cultures of osteosarcoma cells in a microfluidic chip and plastic bag. Cells in microfluidic chips and glass bottom dishes were compared in terms of cell health, cell length, and density. SAOS-2 cells in hypoxic culture had an average length of 18.6 µm for PDMS chips, while in all other cases, the average cell length was approximately 80 µm. Cell density also showed similar results with PDMS chips under hypoxia having the lowest cell density of 30 cells/mm2. The results indicated that the PDMS material was effective in replicating a hypoxic condition of the tumor microenvironment compared to the traditional approach. This study using a PDMS chip and plastic bag serves as a proof of concept for a lab-on-a-chip approach to evaluate tumor growth factors that mimic the tumor environment.</p>
Journal
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- IEEJ Transactions on Sensors and Micromachines
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IEEJ Transactions on Sensors and Micromachines 144 (5), 94-99, 2024-05-01
The Institute of Electrical Engineers of Japan
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Details 詳細情報について
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- CRID
- 1390299993932741632
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- NII Book ID
- AN1052634X
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- ISSN
- 13475525
- 13418939
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- NDL BIB ID
- 033520731
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
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- Abstract License Flag
- Disallowed