The Regulatory Mechanism of <i>Smilax China</i> L. Saponins against Nonalcoholic Fatty Liver Is Revealed by Metabolomics and Transcriptomics

DOI Web Site 参考文献33件
  • Li Cuiyu
    Metabolomics Laboratory, Guangxi Medical University
  • Fang Bin
    Metabolomics Laboratory, Guangxi Medical University
  • Wei Yuanyuan
    Metabolomics Laboratory, Guangxi Medical University
  • Mo Rou
    Metabolomics Laboratory, Guangxi Medical University
  • Lin Xing
    Metabolomics Laboratory, Guangxi Medical University
  • Huang Quanfang
    The Pharmaceutical Department, Guangxi University of Chinese Medicine First Affiliated Hospital

抄録

<p>This study was to investigate the effects of Smilax China L. saponins (SCS) on non-alcoholic fatty liver disease (NAFLD). Rats were fed a high-fat diet (HFD) for 8 weeks to induce NAFLD, followed by SCS treatment for 8 weeks. The effect of SCS on liver injury was observed by H&E staining and the regulative mechanism of SCS on lipid formation was exposed by detecting Oil red O, insulin resistance (IR), and fatty acids synthesis (FAS). Furthermore, transcriptomics and metabolomics were performed to analyze the potential targets. The experimental results indicated that SCS exerted a positive curative effect in alleviating HFD-induced overweight, hepatic injury, steatosis, and lipid formation and accumulation in rats, and the preliminary mechanism studies showed that SCS could alleviate IR, inhibit FAS expression, and reduce Acetyl-CoA levels. Besides, the integrative analysis of transcriptomics and metabolomics exposed the targets of SCS to regulate lipid production likely being the sphingolipid metabolism and glycerophospholipid metabolism pathways. This study demonstrates that SCS significantly ameliorates lipid metabolic disturbance in rats with NAFLD by relieving insulin resistance, inhibiting the FAS enzymes, and regulating the sphingolipid and glycerophospholipid metabolism pathways.</p>

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