Modulations of the mTORC2–GATA3 axis by an isorhamnetin activated endosomal–lysosomal system of the J774.1 macrophage-like cell line

DOI Web Site 参考文献32件 オープンアクセス
  • Sakai Maiko
    Department of Clinical Nutrition and Food Management, Institute of Biomedical Sciences, Tokushima University Graduate School of Medical Nutrition
  • Ohnishi Kohta
    Department of Clinical Nutrition and Food Management, Institute of Biomedical Sciences, Tokushima University Graduate School of Medical Nutrition
  • Masuda Masashi
    Department of Clinical Nutrition and Food Management, Institute of Biomedical Sciences, Tokushima University Graduate School of Medical Nutrition
  • Harumoto Erika
    Department of Clinical Nutrition and Food Management, Institute of Biomedical Sciences, Tokushima University Graduate School of Medical Nutrition
  • Fukuda Teppei
    Department of Clinical Nutrition and Food Management, Institute of Biomedical Sciences, Tokushima University Graduate School of Medical Nutrition
  • Ohnishi Aika
    Department of Clinical Nutrition and Food Management, Institute of Biomedical Sciences, Tokushima University Graduate School of Medical Nutrition
  • Ishii Shunsuke
    Department of Biology, Graduate School of Science and Engineering, Chiba University
  • Ohminami Hirokazu
    Department of Clinical Nutrition and Food Management, Institute of Biomedical Sciences, Tokushima University Graduate School of Medical Nutrition
  • Yamanaka-Okumura Hisami
    Department of Clinical Nutrition and Food Management, Institute of Biomedical Sciences, Tokushima University Graduate School of Medical Nutrition Department of Food Science and Nutrition, Doshisha Women College of Liberal Arts
  • Ohashi Kazuto
    Institute for Molecular and Cellular Regulation, Gunma University
  • Itakura Eisuke
    Department of Biology, Graduate School of Science, Chiba University
  • Horikawa Kazuki
    Department of Optical Imaging, Advanced Research Promotion Center, Tokushima University Graduate School of Medical Nutrition
  • Yonemura Shigenobu
    Department of Cell Biology, Tokushima University Graduate School of Medical Nutrition Laboratory for Ultrastructural Research, Riken Center for Biosystems Dynamics Research
  • Hara Taichi
    Laboratory of Food and Life Science, Faculty of Human Sciences, Waseda University
  • Taketani Yutaka
    Department of Clinical Nutrition and Food Management, Institute of Biomedical Sciences, Tokushima University Graduate School of Medical Nutrition

書誌事項

タイトル別名
  • Modulations of the mTORC2–GATA3 axis by ‍an ‍isorhamnetin activated endosomal–lysosomal system of the J774.1 macrophage-like cell line

説明

<p>The endosomal–lysosomal system represents a crucial degradation pathway for various extracellular substances, and its dysfunction is linked to cardiovascular and neurodegenerative diseases. This degradation process involves multiple steps: (1) the uptake of extracellular molecules, (2) transport of cargos to lysosomes, and (3) digestion by lysosomal enzymes. While cellular uptake and lysosomal function are reportedly regulated by the mTORC1–TFEB axis, the key regulatory signal for cargo transport remains unclear. Notably, our previous study discovered that isorhamnetin, a dietary flavonoid, enhances endosomal–lysosomal proteolysis in the J774.1 cell line independently of the mTORC1–TFEB axis. This finding suggests the involvement of another signal in the mechanism of isorhamnetin. This study analyzes the molecular mechanism of isorhamnetin using transcriptome analysis and reveals that the transcription factor GATA3 plays a critical role in ‍enhanced endosomal–lysosomal degradation. Our data also demonstrate that mTORC2 regulates GATA3 nuclear translocation, and the mTORC2–GATA3 axis alters endosomal formation and maturation, facilitating the efficient transport of cargos to lysosomes. This study suggests that the mTORC2–GATA3 axis might be a novel target for the degradation of abnormal substances.</p>

収録刊行物

参考文献 (32)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ