Automated Tape-Collecting System Ultra-Microtome (ATUM) for Three-Dimensional Reconstruction of Biological Tissue

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  • Iwasaki Hirohide
    Department of Cellular Neurobiology, Graduate School of Medicine, The University of Tokyo

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  • 超薄連続切片自動回収機ATUMを用いた試料作製法
  • チョウハクレンゾク セッペン ジドウ カイシュウキ ATUM オ モチイタ シリョウ サクセイホウ

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Abstract

<p>Recently, technologies for three-dimensional reconstruction of biological samples have developed rapidly. Especially, various methods using scanning electron microscopy have emerged. ATUM (Automated Tape-Collecting system Ultra-Microtome) is one of these technologies and ultrathin sections are collected automatically by using ATUM. In this article, I will introduce the detail of ATUM and will describe how to use this equipment, including the application for light microscopy.</p>

Journal

  • KENBIKYO

    KENBIKYO 49 (3), 176-180, 2014-12-30

    The Japanese Society of Microscopy

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