Gliding Machinery of <i>Mycoplasma mobile</i> Visualized by High-speed AFM

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Other Title
  • 高速AFMが捕らえた!<i>Mycoplasma mobile</i>の滑走装置
  • 高速AFMが捕らえた! Mycoplasma mobileの滑走装置
  • コウソク AFM ガ トラエタ! Mycoplasma mobile ノ カッソウ ソウチ

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Description

<p>Mycoplasma mobile, a fish pathogenic bacterium lacking a peptidoglycan layer glides on a host cell surface with a unique mechanism. In the gliding mechanism, the force is generated through ATP hydrolysis on a gliding motor, evolved from F-type ATPase. We visualized the structural changes of gliding motors in living cells by using high-speed atomic force microscopy (high-speed AFM) which is a unique method to directly observe the dynamic molecules. The high-speed AFM visualized particle structures forming chains at intervals about 30 nm in the cell, consistent with the gliding motor observed by electron microscopy. Half of particles showed 8 nm shift to the left side relative to the gliding direction of cell and 5 nm sink to the cytoplasm side. The others stayed static through the observation. The frequency of the movement was reduced by sodium azide, F-type ATPase inhibitor, suggesting that the movement depends on ATP hydrolysis. In the gliding mechanism, the movement should be converted and amplified to the step of “leg” through “crank”, respectively composed of Gli349 and Gli521 on cell surface.</p>

Journal

  • KENBIKYO

    KENBIKYO 54 (2), 67-71, 2019-08-30

    The Japanese Society of Microscopy

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