Quantitative Identification of Soybean Cultivars Using Digital PCR

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  • デジタルPCRを用いたダイズおよびその加工品の定量的品種判別法の開発
  • デジタル PCR オ モチイタ ダイズ オヨビ ソノ カコウヒン ノ テイリョウテキ ヒンシュ ハンベツホウ ノ カイハツ

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Abstract

<p>A quantitative method to identify soybean cultivars was developed using the digital polymerase chain reaction (PCR) in order to ensure reliability of food labelling. Primers and probes were designed for specific detection of the cultivars “Fukuyutaka” and “Enrei”, the most used Japanese cultivars for Tofu production. As a result of analysis using a DNA mixed sample of both cultivars extracted from raw soybeans and Tofu, the digital PCR method that was developed showed a strong correlation between measured values and expected values, as well as accurate quantification of small amounts of contamination.</p>

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