Development of an Educational Material for Teaching “Central Dogma” in the High School Biology -Visualization of Transcription and Translation Process Reconstituted in a Test Tube-

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  • Katayama T.
    群馬県立渋川女子高等学校 愛媛大学無細胞生命科学工学研究センター
  • Hayashi H.
    愛媛大学無細胞生命科学工学研究センター 愛媛大学大学院理工学研究科
  • Takai K.
    愛媛大学無細胞生命科学工学研究センター 愛媛大学大学院理工学研究科
  • Endo Y.
    愛媛大学無細胞生命科学工学研究センター 愛媛大学大学院理工学研究科

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Other Title
  • セントラルドグマを体感する高等学校生物実験の開発と実践
  • セントラルドグマを体感する高等学校生物実験の開発と実践 : コムギ胚芽無細胞タンパク質合成系を用いて転写,翻訳を可視化する
  • セントラルドグマ オ タイカン スル コウトウ ガッコウ セイブツ ジッケン ノ カイハツ ト ジッセン : コムギハイガ ムサイボウ タンパクシツ ゴウセイケイ オ モチイテ テンシャ,ホンヤク オ カシカ スル
  • ―コムギ胚芽無細胞タンパク質合成系を用いて転写，翻訳を可視化する―

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<p>In the high school curriculum today the concept of“The central dogma” is not included in“Biology”. Only students who take the applied biology class have an opportunity to learn the concept in “BiologyⅡ”, where an E. coli cell-based recombinant system has been used for a student experiment. In the new curriculum coming in 2012, it is scheduled to be taught the central dogma at first or second grade in the first chapter of “Basic Biology”. In view of the importance in modern biology, it has been desired to have a new educational material which hits the spots in both features, effectiveness and easy-handling. Here we introduced that an in vitro simple gene expression kit is useful for visualization of transcription and translation processes in a test tube. Transcription was carried out using a phage RNA polymerase, and production of the mRNA was directly monitored using an RNA staining dye. Green fluorescent protein (GFP) was translated in the wheat germ cell- free protein synthesis system, and the product was detected directly by its fluorescence. Having established the kit, we have tested its value in the present “BiologyⅡ” classes, and could in fact confirm its usefulness. Since wheat seeds are foodstuff, the kit is free of bio-hazard and ethical issues. We expect that the kit will provide effective methods also in the new curriculum.</p>

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