Artificial photosynthetic cell producing energy for protein synthesis

DOI

Bibliographic Information

Other Title
  • 光依存的にエネルギーを生産する人工細胞の構築

Abstract

<p>Energy production is one of the remarkable characteristics of life. Within a cell, biological energy, adenosine triphosphate (ATP), is produced by ATP synthase, which is a membrane protein machinery, using pre-formed membrane potential. Here, we constructed an artificial organelle (AO) using ATP synthase and a photoreactive proton-pump, bacteriorhodopsin (bR). ATP synthase and bR were integrated in a small size liposome. The resulting AO generates a proton gradient by light and drove ATP synthase. The AO produced ATP even inside a micro size vesicle in few mM level. So photosynthesized ATP was consumed as an energy for protein synthesis by coupling with a cell-free protein synthesis system. Interestingly, we also success to photosynthesize parts of AO and observed that the activity of AO was enhanced after the photosynthesis reaction. This result represents that the artificial cell can produce own component by using self-sufficient energy, in the scheme of positive feedback loop.</p>

Journal

Details 詳細情報について

  • CRID
    1390564227328544512
  • NII Article ID
    130007737771
  • DOI
    10.14886/jvss.2019.0_3dp04
  • ISSN
    24348589
  • Text Lang
    ja
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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