Continuous Separation of Micro-particles by Microchip Electromagnetophoresis Utilizing Aqueous Two-phase Flow

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  • IIGUNI Yoshinori
    Department of Life Science and Applied Chemistry, Graduate School of Engineering, Nagoya Institute of Technology
  • TAMURA Reo
    Department of Life Science and Applied Chemistry, Graduate School of Engineering, Nagoya Institute of Technology
  • KITAGAWA Shinya
    Department of Life Science and Applied Chemistry, Graduate School of Engineering, Nagoya Institute of Technology
  • OHTANI Hajime
    Department of Life Science and Applied Chemistry, Graduate School of Engineering, Nagoya Institute of Technology

Bibliographic Information

Other Title
  • 水性二相流を利用するマイクロチップ電磁泳動法による微粒子の連続分離
  • スイセイ ニソウリュウ オ リヨウ スル マイクロチップ デンジ エイドウホウ ニ ヨル ビリュウシ ノ レンゾク ブンリ

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Abstract

<p>We report on a novel control technique for the transport of microparticles in a micro-channel for microchip analysis using electromagnetophoresis combined with a conductive/non-conductive aqueous two phase (ATP) flow. A double Y-shaped microchip (20 mm length, 160 μm width) with two inlets and two outlets was used to form a conductive/non-conductive ATP flow. When a 1.16 mol L−1 KCl aqueous solution and a 20 % dextran (Dex) aqueous solution were used as a conductive fluid and a non-conductive fluid, respectively, a conductive/non-conductive ATP flow could be formed in the micro-channel. In this conductive/non-conductive ATP flow, polystyrene (PS) particles with 3, 6 and 10 μm diameters dispersed in the conductive fluid could be focused on the ATP flow interface when electromagnetophoresis was applied on the particles toward the ATP flow interface. On the other hand, carboxylate PS (cPS) particles with 10 μm diameter were transferred into a Dex solution in the same situation. Moreover, by applying staggered-electromagnetophoresis, PS particles were redistributed in the conductive fluid although cPS particles stayed in the non-conductive fluid. Consequently, PS particles and cPS particles could be eluted at different outlets, respectively.</p>

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 68 (10), 743-750, 2019-10-05

    The Japan Society for Analytical Chemistry

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