Electrochemical Flow Immunoassay Using Capillary Column and Ferrocene Conjugated Immunoglobulin G

DOI Web Site Web Site 25 References Open Access
  • LIM Tae-Kyu
    Department of Biotechnology, Tokyo University of Agriculture and Technology
  • NAKAMURA Noriyuki
    National Institute for Advanced Interdisciplinary Research
  • IKEHATA Masateru
    Department of Biotechnology, Tokyo University of Agriculture and Technology
  • MATSUNAGA Tadashi
    Department of Biotechnology, Tokyo University of Agriculture and Technology

Search this article

Description

<p>This paper describes a miniaturized flow immunoassay system. Ferrocene (Fc) conjugated with anti-HCG immunoglobulin G (IgG) antibody (Fc-IgG) was prepared and used as a novel analytical reagent. The system consists of the immunoreaction part, the capillary column packed with cation exchange resin, and the flow cell for electrochemical detection of Fc-IgG. The assay yielded a linear relationship between signal and HCG concentration in the range of 0-2000 mIU/mL (L=dm3). This electrochemical flow immunoassay requires minute quantities of serum and generates highly reproducible results.</p>

Journal

  • Electrochemistry

    Electrochemistry 68 (11), 872-874, 2000-11-05

    The Electrochemical Society of Japan

References(25)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top