イメージングフローサイトメーターを用いた新規T細胞機能検査の開発

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  • Development of new T cell function test using imaging flow cytometer

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<p>T cells play a central role in cell-mediated immunity. Abnormalities in T cells have been reported in a number of diseases and several drugs that alter T cell activity have emerged in the fields of cancer, autoimmune diseases, allergies, and others. The need for T cell function tests has increased; however, the long measurement time and low reproducibility of these tests are a major concern. </p><p>Recent studies have shown that T cells induce receptor aggregation to form an immune synapse (IS) within two minutes of stimulation. Theoretically, IS formation within a short time. Here, we attempted to establish a rapid and accurate T cell function test based on this principle.</p><p></p><p>We used human monoclonal T cells, which secrete interferon gamma (IFN-γ) via antibody stimulation, as a stimulus-responsive T cell control. We examined various conditions that determined the induction of IS formation and detection of the formed IS. As a result, we induced IS formation and detected it in monoclonal T cells. Furthermore, the IS formation rate correlated with the results of the existing cytokine flow cytometry assay. Using this method, we measured the stimulus response of blood mononuclear cells and observed that the variation was less than 10%. Based on these results, we believe that the principle of a new T cell function test has been established.</p>

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