Comparative Analysis of Immune Responses to Outer Membrane Antigens OMP10, OMP19, and OMP28 of Brucella abortus
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- Im Young Bin
- Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University
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- Park Woo Bin
- Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University
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- Jung Myunghwan
- Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University
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- Kim Suk
- College of Veterinary Medicine, Gyeongsang National University
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- Yoo Han Sang
- Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University Institute of Green Bio Science and Technology, Seoul National University
書誌事項
- タイトル別名
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- Comparative Analysis of Immune Responses to Outer Membrane Antigens OMP10, OMP19, and OMP28 of <i>Brucella abortus</i>
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抄録
<p>Brucella infection is accompanied by cytokine production, which serves as an important factor to evaluate the innate and adaptive immune responses. Several researchers have been investigating the mechanisms involved in Brucella infection in the host. Here, we conducted an analytical study to define pathogenic pathways and immune mechanisms involved in Brucella infection by investigating the antigenic efficacy of recombinant outer membrane protein 10 (rOMP10), outer membrane protein 19 (rOMP19), and outer membrane protein 28 (rOMP28) in vitro and in vivo upon stimulation/immunization. Cytokine production was analyzed by nitric oxide (NO) assay and enzyme-linked immunosorbent assay (ELISA) after stimulation of RAW 264.7 cells and naive splenocytes with the recombinant proteins. Our results show that levels of NO, tumor necrosis factor (TNF)-α, and interleukin (IL)-6 increased in RAW 264.7 cells in a time-dependent manner following recombinant protein stimulation. In contrast, levels of interferon (IFN)-γ and IL-2 increased in naive splenocytes after stimulation with proteins. ELISA and ELISpot assays were performed after immunization of mice with recombinant proteins. rOMP28 greatly increased IFN-γ, IL-2, and TNF-α levels than IL-4 and IL-6 levels in vitro. Of the recombinant proteins, rOMP19 elicited a mixed Th1/Th2 immune response by increasing the number of IgG-secreting cells in vivo.</p>
収録刊行物
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- Japanese Journal of Infectious Diseases
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Japanese Journal of Infectious Diseases 71 (3), 197-204, 2018
国立感染症研究所 Japanese Journal of Infectious Diseases 編集委員会
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詳細情報 詳細情報について
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- CRID
- 1390564237991198592
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- NII論文ID
- 130006743902
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- NII書誌ID
- AA1132885X
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- ISSN
- 18842836
- 13446304
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- NDL書誌ID
- 029009463
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- PubMed
- 29709972
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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