Biochemical and Morphological Characterization of a Guanine Nucleotide Exchange Factor ARHGEF9 in Mouse Tissues

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  • Ibaraki Kyoko
    Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Human Service Center
  • Mizuno Makoto
    Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Human Service Center
  • Aoki Hitomi
    Department of Tissue and Organ Development, Gifu University Graduate School of Medicine
  • Niwa Ayumi
    Department of Tumor Pathology, Gifu University Graduate School of Medicine
  • Iwamoto Ikuko
    Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Human Service Center
  • Hara Akira
    Department of Tumor Pathology, Gifu University Graduate School of Medicine
  • Tabata Hidenori
    Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Human Service Center
  • Ito Hidenori
    Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Human Service Center
  • Nagata Koh-ichi
    Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Human Service Center Department of Neurochemistry, Nagoya University Graduate School of Medicine

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Abstract

<p>ARHGEF9, also known as Collybistin, a guanine nucleotide exchange factor for Rho family GTPases, is thought to play an essential role in the mammalian brain. In this study, we prepared a specific polyclonal antibody against ARHGEF9, anti-ARHGEF9, and carried out expression analyses with mouse tissues especially brain. Western blotting analyses demonstrated tissue-dependent expression profiles of ARHGEF9 in the young adult mouse, and strongly suggested a role during brain development. Immunohistochemical analyses revealed developmental stage-dependent expression profiles of ARHGEF9 in cerebral cortex, hippocampus and cerebellum. ARHGEF9 exhibited partial localization at dendritic spines in cultured hippocampal neurons. From the obtained results, anti-ARHGEF9 was found to be a useful tool for biochemical and cell biological analyses of ARHGEF9.</p>

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