Biochemical and Morphological Characterization of a Guanine Nucleotide Exchange Factor ARHGEF9 in Mouse Tissues
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- Ibaraki Kyoko
- Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Human Service Center
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- Mizuno Makoto
- Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Human Service Center
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- Aoki Hitomi
- Department of Tissue and Organ Development, Gifu University Graduate School of Medicine
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- Niwa Ayumi
- Department of Tumor Pathology, Gifu University Graduate School of Medicine
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- Iwamoto Ikuko
- Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Human Service Center
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- Hara Akira
- Department of Tumor Pathology, Gifu University Graduate School of Medicine
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- Tabata Hidenori
- Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Human Service Center
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- Ito Hidenori
- Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Human Service Center
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- Nagata Koh-ichi
- Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Human Service Center Department of Neurochemistry, Nagoya University Graduate School of Medicine
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Description
<p>ARHGEF9, also known as Collybistin, a guanine nucleotide exchange factor for Rho family GTPases, is thought to play an essential role in the mammalian brain. In this study, we prepared a specific polyclonal antibody against ARHGEF9, anti-ARHGEF9, and carried out expression analyses with mouse tissues especially brain. Western blotting analyses demonstrated tissue-dependent expression profiles of ARHGEF9 in the young adult mouse, and strongly suggested a role during brain development. Immunohistochemical analyses revealed developmental stage-dependent expression profiles of ARHGEF9 in cerebral cortex, hippocampus and cerebellum. ARHGEF9 exhibited partial localization at dendritic spines in cultured hippocampal neurons. From the obtained results, anti-ARHGEF9 was found to be a useful tool for biochemical and cell biological analyses of ARHGEF9.</p>
Journal
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- ACTA HISTOCHEMICA ET CYTOCHEMICA
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ACTA HISTOCHEMICA ET CYTOCHEMICA 51 (3), 119-128, 2018-06-26
JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY
