Development of a stable Agrobacterium-mediated transformation protocol for Sorghum bicolor Tx430

DOI Web Site Web Site 被引用文献2件 参考文献14件
  • Sato-Izawa Kanna
    Gene Research Center, University of Tsukuba Department of Bioscience, Faculty of Life Sciences, Tokyo University of Agriculture
  • Tokue Kyoko
    Gene Research Center, University of Tsukuba
  • Ezura Hiroshi
    Gene Research Center, University of Tsukuba

書誌事項

タイトル別名
  • Development of a stable <i>Agrobacterium</i>-mediated transformation protocol for <i>Sorghum bicolor</i> Tx430

この論文をさがす

抄録

<p>Sorghum is a recalcitrant crop for Agrobacterium-mediated genetic transformation. Several parameters related to Agrobacterium-mediated transformation were tested to optimize sorghum transformation frequencies. In this study, we evaluated pretreatment of sorghum variety Tx430 immature embryos using Agrobacterium strain GV2260. Pretreatment of immature embryos with heat (43°C) treatment for 15 or 21 min, and centrifugation resulted in a transformation efficiency of up to 1.9% of immature embryos treated. Although further optimization to enhance transformation efficiency is required, this study contributes to the genetic validation of genes of interest and molecular breeding in sorghum plants.</p>

収録刊行物

被引用文献 (2)*注記

もっと見る

参考文献 (14)*注記

もっと見る

関連プロジェクト

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ