Development of a stable Agrobacterium-mediated transformation protocol for Sorghum bicolor Tx430
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- Sato-Izawa Kanna
- Gene Research Center, University of Tsukuba Department of Bioscience, Faculty of Life Sciences, Tokyo University of Agriculture
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- Tokue Kyoko
- Gene Research Center, University of Tsukuba
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- Ezura Hiroshi
- Gene Research Center, University of Tsukuba
書誌事項
- タイトル別名
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- Development of a stable <i>Agrobacterium</i>-mediated transformation protocol for <i>Sorghum bicolor</i> Tx430
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<p>Sorghum is a recalcitrant crop for Agrobacterium-mediated genetic transformation. Several parameters related to Agrobacterium-mediated transformation were tested to optimize sorghum transformation frequencies. In this study, we evaluated pretreatment of sorghum variety Tx430 immature embryos using Agrobacterium strain GV2260. Pretreatment of immature embryos with heat (43°C) treatment for 15 or 21 min, and centrifugation resulted in a transformation efficiency of up to 1.9% of immature embryos treated. Although further optimization to enhance transformation efficiency is required, this study contributes to the genetic validation of genes of interest and molecular breeding in sorghum plants.</p>
収録刊行物
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- Plant Biotechnology
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Plant Biotechnology 35 (2), 181-185, 2018-06-25
日本植物バイオテクノロジー学会
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詳細情報 詳細情報について
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- CRID
- 1390564237996564096
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- NII論文ID
- 130007393758
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- NII書誌ID
- AA11250821
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- ISSN
- 13476114
- 13424580
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- NDL書誌ID
- 029091841
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可