Development of a Highly-Sensitive Two-Dimensional HPLC System with Narrowbore Reversed-Phase and Microbore Enantioselective Columns and Application to the Chiral Amino Acid Analysis of the Mammalian Brain
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- FURUSHO Aogu
- Graduate School of Pharmaceutical Sciences, Kyushu University
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- KOGA Reiko
- Graduate School of Pharmaceutical Sciences, Kyushu University Faculty of Pharmaceutical Sciences, Fukuoka University
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- AKITA Takeyuki
- Graduate School of Pharmaceutical Sciences, Kyushu University
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- MIYOSHI Yurika
- Shiseido Co., Ltd.
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- MITA Masashi
- Shiseido Co., Ltd.
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- HAMASE Kenji
- Graduate School of Pharmaceutical Sciences, Kyushu University
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<p>A highly-sensitive two-dimensional high-performance liquid chromatographic (2D-HPLC) system with narrowbore reversed-phase and microbore enantioselective columns has been developed for the determination of alanine (Ala) and serine (Ser) enantiomers. The amounts of D-amino acids in biological samples are extremely low in most cases and improvement in the sensitivity is still required. For a highly-sensitive analysis, a narrowbore reversed-phase column, KSAARP (1.5 mm x 500 mm, having a wider ID than the microbore column) was used in the first dimension, and a microbore enantioselective column, KSAACSP-001S (1.0 mm x 250 mm, having a narrower ID than the frequently reported enantioselective columns) was used in the second dimension. The amino acids were derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole and detected by the fluorescence detectors. The 2D-HPLC system was validated using a mouse cerebrum in addition to the standard amino acids, and satisfactory calibration lines and precision results were obtained. In the mouse cerebrum, a large amount of D-Ser and a small amount of D-Ala were clearly observed. These results indicated that the present method is applicable to the determination of the trace amounts of D-amino acids in tissue samples.</p>
収録刊行物
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- CHROMATOGRAPHY
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CHROMATOGRAPHY 39 (2), 83-90, 2018-06-20
クロマトグラフィー科学会
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詳細情報 詳細情報について
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- CRID
- 1390564237996747264
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- NII論文ID
- 130007403273
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- NII書誌ID
- AA1137755X
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- ISSN
- 13483315
- 13428284
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- NDL書誌ID
- 029139056
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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