Determination of Histamine by a Rapid Fluorescamine-UPLC Method

DOI JASI Web Site Web Site PubMed 2 Citations 4 References

Bibliographic Information

Other Title
  • フルオレスカミン蛍光誘導体化UPLC法を用いたヒスタミン迅速分析法
  • フルオレスカミン ケイコウ ユウドウタイカ UPLCホウ オ モチイタ ヒスタミン ジンソク ブンセキホウ

Search this article

Abstract

<p>We have developed a rapid analysis method for determination of histamine using ultra-performance liquid chromatography and fluorescamine derivatization. Histamine was extracted from foods with trichloroacetic acid after homogenization. The supernatant obtained by centrifugation was filtered and derivatized without solid-phase extraction. The mobile phase was 10 mM phosphate–acetonitrile (75 : 25) without ion-pairing. Recovery tests of histamine from fishes, seasoning and fish products gave acceptable recovery (95.8–117.7%). This method should be useful for rapid evaluation of food poisoning outbreaks.</p>

Journal

Citations (2)*help

See more

References(4)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top