書誌事項
- タイトル別名
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- Direct High-Pressure NMR Observation of Molecular Processes of Spore Inactivation by a Sequential Pressure-Temperature Perturbation
- アツリョク-オンド ショリ ニ ヨル ガホウ シメツ ノ コウアツ NMR チョクセツ カンソク
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Some bacteria such as the Bacillus and Clostridium species can form spores, which are the dormant form of vegetative bacteria and are highly resistant to physical and chemical stimuli, the sterilization of which requires heating at ~121°C for 30 min. A crucial factor that maintains this high-resistivity is the high concentration (~10% in dry weight of spore) of dipicolinic acid (DPA), which is spore-specific. We have applied 1H high-resolution high-pressure NMR spectroscopy, for the first time, to the spore suspension, and observed, directly and in real-time, the specific release of DPA molecules from the spore into the bulk water phase in response to a mild hydrostatic pressure (200 MPa) at 20°C. After the release of DPA, heat-treatment below ~100°C has proven to be sufficient to cause the inactivation of the spores. Direct observation of molecular processes in bacterial spores with high-pressure NMR in response to pressure and temperature perturbations shown here opens a new avenue for studying spores and vegetative cells directly at the molecular level.<br>
収録刊行物
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- 高圧力の科学と技術
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高圧力の科学と技術 28 (2), 113-122, 2018
日本高圧力学会
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詳細情報 詳細情報について
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- CRID
- 1390564237998859264
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- NII論文ID
- 130007402300
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- NII書誌ID
- AN10452913
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- ISSN
- 13481940
- 0917639X
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- NDL書誌ID
- 029179156
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可