Quantification of Organic Acids in Foods by Flow Injection Analysis Using Immobilized Enzyme Reactor

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  • 固定化酵素リアクターを用いたフローインジェクション分析法による食品中の有機酸類の定量
  • コテイカ コウソ リアクター オ モチイタ フローインジェクション ブンセキホウ ニ ヨル ショクヒン チュウ ノ ユウキ サンルイ ノ テイリョウ
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Abstract

Organic acids affect the taste and flavor of foods, influencing our perception of sourness and freshness in harmony with sweetness and bitterness. Thus, a method for the quantification of organic acids in foods by flow injection analysis (FIA) using an immobilized enzyme reactor was developed. FIA systems for L-malate, D-malate, L-tartrate, citrate, isocitrate, succinate, D-gluconate, pyruvate, L-lactate, acetate, γ-aminobutyrate, and L-glutamate were established and applied to the quantification of component in the foods. The results obtained by the proposed FIA systems were in good agreement with those obtained by conventional methods (HPLC or F-kit method). The proposed FIA systems are useful flow methods for the quantification of organic acids in foods and are useful in judging the quality of food and quality control of the fermentation process.

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