Developments in Protein Assays with Flow Injection/Sequential Injection Techniques

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Abstract

The development of flow-based techniques, including flow injection and sequential injection analysis, offers an excellent on-line alternative with respect to conventional wet chemical assays with the merits of promoted automation, reduced sample/reagent consumption, minimized sample contamination, improved accuracy and analysis throughput with programmable fluidic manipulations. The superb compatibility to external equipments, designable flow manifolds and enclosed analytical environment make flow-based technique a promising potential platform for the bioassays. In the present mini-review, the up-to-date applications of flow injection/sequential injection approaches in protein assays including automated quantification, on-line qualitative separation, adsorption and extraction, activity investigations and immobilization studies are summarized, and future perspectives are discussed.

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