Correlation of genetic diversity between hosts and parasites in Entamoeba nuttalli isolates from Tibetan and rhesus macaques in China
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- Wei Miao
- Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University
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- Feng Meng
- Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University
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- Guan Yue
- Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University
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- Guo Ce
- Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University
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- Zhou Hang
- Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University
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- Fu Yongfeng
- Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University
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- Tachibana Hiroshi
- Department of Infectious Diseases, Tokai University School of Medicine
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- Cheng Xunjia
- Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University Department of Infectious Diseases, Tokai University School of Medicine
書誌事項
- タイトル別名
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- Correlation of genetic diversity between hosts and parasites in <i>Entamoeba nuttalli</i> isolates from Tibetan and rhesus macaques in China
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説明
<p>Entamoeba nuttalli infection is prevalent in captive and wild macaques. Recent studies have suggested that genotypes of E. nuttalli isolates are correlated with the geographical distribution of host macaques. Correlation of amoebic genotypes with genetic diversity of host macaques was analyzed in present study. Sixty fresh stool samples were obtained from wild Tibetan macaques living in Mount Huang (HS) of the An-hui Province in China. PCR analysis revealed that the most prevalent Entamoeba species was E. chattoni (E. polecki ST2) (86.7%) followed by E. nuttalli (58.3%) and E. coli (25%). Six E. nuttalli HS isolates were successfully cultured. The tRNA-linked short tandem repeat (STR) loci and serine-rich protein gene of E. nuttalli isolates from four different regions of China (Mount Long-hu, Gui-yang, Mount E-mei, and HS, the former three isolates were obtained in previous studies) were studied and high numbers of polymorphisms were detected. When genetic diversity of different populations of E. nuttalli isolates was compared with geographical distance, an r2 value of 0.919 was assigned by a Mantel test based on the tRNA-STR loci. In host macaques, the mtDNA HVS-I gene was also highly polymorphic in each of the genomes. Multiple regression analysis using E. nuttalli tRNA-STR loci genetic, macaque mtDNA HVS-I gene, and geographic distances showed an r2 value of 0.943, indicating that a higher relevance was demonstrated when geographic and host gene factors were considered. Analysis of genetic factor of host would benefit for better understanding of the evolution of E. nuttalli.</p>
収録刊行物
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- BioScience Trends
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BioScience Trends 12 (4), 375-381, 2018-08-31
特定非営利活動法人 バイオ＆ソーシャル・サイエンス推進国際研究交流会
