Transcriptional analysis of alcohol and aldehyde dehydrogenase gene families in <i>Pholiota microspora</i> , and estimation of their physiological roles

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  • <i>Pholiota microspora</i> におけるアルコールと アルデヒド脱水素酵素遺伝子ファミリー の転写分析，その生理的役割の推定
  • Transcriptional analysis of alcohol and aldehyde dehydrogenase gene families in Pholiota microspora, and estimation of their physiological roles

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In this study, we analyzed two alcohol dehydrogenase (Adh), eight aldehyde dehydrogenase (Aldh) and two mannitol-1-phosphate dehydrogenase (Mpd) genes in Pholiota microspora. The transcription of Aldh1, 2, 3, Adh1, 2, and Mpd1, 2 was unaffected in liquid medium in the presence of 1 mM ethanol. However, Mpd1 expression was promoted by acetaldehyde. Therefore, Mpd1 is a candidate for an ethanol-producing alcohol dehydrogenase. On the other hand, transcription of Aldh1 and Adh2 was promoted with 3 mM veratryl alcohol; therefore, the role of Aldh1 and Adh2 might have evolved from alcohol metabolism to degradation of aromatic compounds. Transcription of Adh1, Mpd1, Mpd2 and Aldh1 was higher in primordia and fruiting bodies than mycelia. This phenomenon suggested that the response of Adh1, Mpd1, Mpd2 and Aldh1 indicates the presence of oxidative stress during fruiting body development.

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