Determination of trace amounts of chiral amino acids in complicated biological samples using two-dimensional high-performrance liquid chromatography with an innovative “shape-fitting” peak identification/quantification method

DOI Web Site Web Site 被引用文献9件 参考文献29件

書誌事項

タイトル別名
  • Determination of Trace Amounts of Chiral Amino Acids in Complicated Biological Samples Using Two-Dimensional High-Performance Liquid Chromatography with an Innovative “Shape-Fitting” Peak Identification/Quantification Method

この論文をさがす

抄録

<p>A new peak identification/quantification method, i.e., “shape-fitting” method, has been devised and applied to the determination of chiral amino acids in human plasma and urine. The shape fitting method enables the determination of target analytes using a part of the peak by mathematically predicting the whole peak even when the baseline is not clear. Using this method, D-glutamic acid (Glu) and D-proline (Pro) as well as their L-enantiomers in the plasma and urine were determined. The calibration lines of 4 target enantiomers were linear with correlation coefficients higher than 0.9998; the RSD values of the intra-day precision and inter-day precision were less than 6.70%. In the human plasma, trace amount of D-Pro (0.47 nmol/mL) was observed, and in the urine, 2.97 nmol/mL of D-Glu and 0.08 nmol/mL of D-Pro were successfully determined.</p><p></p>

収録刊行物

  • CHROMATOGRAPHY

    CHROMATOGRAPHY 39 (3), 147-152, 2018-10-20

    クロマトグラフィー科学会

被引用文献 (9)*注記

もっと見る

参考文献 (29)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ