Isolation and purification of extracellular deoxyribonuclease from <i>Streptococcus sanguis</i> I

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  • Hamada Ikuo
    Department of Microbiology, School of Dentistry, Iwate Medical University
  • Honda Hisako
    Department of Microbiology, School of Dentistry, Iwate Medical University
  • Tajika Shihoko
    Department of Microbiology, School of Dentistry, Iwate Medical University
  • Yanagihara Takashi
    Department of Microbiology, School of Dentistry, Iwate Medical University
  • Kaneko Masaru
    Department of Microbiology, School of Dentistry, Iwate Medical University

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  • <i>Streptococcus sanguis</i> I DNase の分離精製

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<p>DNase was separated from culture fluid of Streptococcus sanguis I and another biochemical properties of DNase were examined.</p><p>The enzymatic activity was purified about 6 fold by DEAE-Sephadex A-50 ion exchange chromatography and further Gelfiltration on a Sephadex G-100 column. Two DNase which designated fr. a and fr. b were fractionated. Molecular weights of these enzyme were determined by Sephadex G-50 gelchromatography, fr. a is 24,000 apporoximately and fr. b is 8,000.</p><p>Optimal pH range is 7.0~9.0, there are double peak. This enzyme was activated by the addition of Mg ion, but illhibited by Ca ion.</p><p>On the other hand, the enzyme has not activity for RNA (Yeast) and has specific activity for native DNA. The thermally denatured DNA is poor substrate, 30% of activity decreased.</p>

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