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Isolation and purification of extracellular deoxyribonuclease from <i>Streptococcus sanguis</i> I
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- Hamada Ikuo
- Department of Microbiology, School of Dentistry, Iwate Medical University
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- Honda Hisako
- Department of Microbiology, School of Dentistry, Iwate Medical University
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- Tajika Shihoko
- Department of Microbiology, School of Dentistry, Iwate Medical University
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- Yanagihara Takashi
- Department of Microbiology, School of Dentistry, Iwate Medical University
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- Kaneko Masaru
- Department of Microbiology, School of Dentistry, Iwate Medical University
Bibliographic Information
- Other Title
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- <i>Streptococcus sanguis</i> I DNase の分離精製
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Description
<p>DNase was separated from culture fluid of Streptococcus sanguis I and another biochemical properties of DNase were examined.</p><p>The enzymatic activity was purified about 6 fold by DEAE-Sephadex A-50 ion exchange chromatography and further Gelfiltration on a Sephadex G-100 column. Two DNase which designated fr. a and fr. b were fractionated. Molecular weights of these enzyme were determined by Sephadex G-50 gelchromatography, fr. a is 24,000 apporoximately and fr. b is 8,000.</p><p>Optimal pH range is 7.0~9.0, there are double peak. This enzyme was activated by the addition of Mg ion, but illhibited by Ca ion.</p><p>On the other hand, the enzyme has not activity for RNA (Yeast) and has specific activity for native DNA. The thermally denatured DNA is poor substrate, 30% of activity decreased.</p>
Journal
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- Dental Journal of Iwate Medical University
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Dental Journal of Iwate Medical University 7 (2), 124-130, 1982-07-15
The Dental Society of Iwate Medical University
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Details 詳細情報について
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- CRID
- 1390564238058404480
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- NII Article ID
- 130007552493
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- ISSN
- 24241822
- 03851311
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- Web Site
- http://id.nii.ac.jp/1181/00002842/
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- Text Lang
- ja
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- Article Type
- departmental bulletin paper
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- Data Source
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- JaLC
- IRDB
- CiNii Articles
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- Abstract License Flag
- Disallowed