Rabbit induced pluripotent stem cells retain capability of in vitro cardiac differentiation

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  • Phakdeedindan Praopilas
    Biochemistry Unit, Department of Physiology, Faculty of Veterinary Science, Chulalongkorn University, 39 Henri-Dunant Rd., Pathumwan, Bangkok 10330, Thailand
  • Setthawong Piyathip
    Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, 39 Henri-Dunant Rd., Pathumwan, Bangkok 10330, Thailand
  • Tiptanavattana Narong
    Faculty of Veterinary Science, Prince of Songkla University, 15 Kanjanavanich Road, Hat Yai Songkhla 90110, Thailand
  • Rungarunlert Sasitorn
    Faculty of Veterinary Science, Mahidol University, 999 Phutthamonthon Sai 4 Road, Nakhonpathom, 73170, Thailand
  • Ingrungruanglert Praewphan
    Stem Cells and Cell Therapy Research Unit, Faculty of Medicine, Chulalongkorn University, 1873 Henri-Dunant Rd., Pathumwan, Bangkok 10330, Thailand
  • Israsena Nipan
    Stem Cells and Cell Therapy Research Unit, Faculty of Medicine, Chulalongkorn University, 1873 Henri-Dunant Rd., Pathumwan, Bangkok 10330, Thailand
  • Techakumphu Mongkol
    Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, 39 Henri-Dunant Rd., Pathumwan, Bangkok 10330, Thailand
  • Tharasanit Theerawat
    Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, 39 Henri-Dunant Rd., Pathumwan, Bangkok 10330, Thailand The Research and Development Center for Livestock Production Technology at the Faculty of Veterinary Science, Chulalongkorn University, Thailand

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タイトル別名
  • Rabbit induced pluripotent stem cells retain capability of <i>in vitro</i> cardiac differentiation

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<p>Stem cells are promising cell source for treatment of multiple diseases as well as myocardial infarction. Rabbit model has essentially used for cardiovascular diseases and regeneration but information on establishment of induced pluripotent stem cells (iPSCs) and differentiation potential is fairly limited. In addition, there is no report of cardiac differentiation from iPSCs in the rabbit model. In this study, we generated rabbit iPSCs by reprogramming rabbit fibroblasts using the 4 transcription factors (OCT3/4, SOX2, KLF4, and c-Myc). Three iPSC lines were established. The iPSCs from all cell lines expressed genes (OCT3/4, SOX2, KLF4 and NANOG) and proteins (alkaline phosphatase, OCT-3/4 and SSEA-4) essentially described for pluripotency (in vivo and in vitro differentiation). Furthermore, they also had ability to form embryoid body (EB) resulting in three-germ layer differentiation. However, ability of particular cell lines and cell numbers at seeding markedly influenced on EB formation and also their diameters. The cell density at 20,000 cells per EB was selected for cardiac differentiation. After plating, the EBs attached and cardiac-like beating areas were seen as soon as 11 days of culture. The differentiated cells expressed cardiac progenitor marker FLK1 (51 ± 1.48%) on day 5 and cardiac troponin-T protein (10.29 ± 1.37%) on day 14. Other cardiac marker genes (cardiac ryanodine receptors (RYR2), α-actinin and PECAM1) were also expressed. This study concluded that rabbit iPSCs remained their in vitro pluripotency with capability of differentiation into mature-phenotype cardiomyocytes. However, the efficiency of cardiac differentiation is still restricted.</p>

収録刊行物

  • Experimental Animals

    Experimental Animals 68 (1), 35-47, 2019

    公益社団法人 日本実験動物学会

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