培養骨芽細胞に対する重畳した機械的振動の影響

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  • The Effects of Superposed Mechanical Vibration on Cultured Osteoblasts

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<p>The previous study showed that the mechanical vibrations at 12.5 Hz and 50 Hz promote cell proliferation and differentiation, respectively. If the response of cells to mechanical vibration is linear, the superposed mechanical vibration at 12.5 Hz and 50 Hz would activate both of them. However, we do not fully understand cell systems. It is necessary to conduct experiments to investigate the effects of the superposed vibration. Therefore, the objective of this study is to investigate the effects of superposed mechanical vibration on cell proliferation and differentiation experimentally. Osteoblast-like cells were culture for the non-vibration groups and the vibration groups. The vibration groups were cultured under the vertical superposed vibration for 24h/day. The vibration was made by a shaker which was operated by a signal from a function generator. The vibration consisted of the 12.5 Hz and 0.5 G sine wave and the 50 Hz and 0.5 G sine wave. The number of cells, the area of mineralization, and the ALP gene expression level were obtained by cell counting with a hemocytometer, alizarin red staining, and real time RT-PCR, respectively. As a result, there was no significant difference in the ALP gene expression level, but the maximum value of cell density was 2.8 times larger and the mineralization appeared earlier in the vibration groups. From the above, the superposed vibration has effects of the cell proliferation and there is possibility of the effects of the vibration on cell differentiation.</p>

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