Dipeptide synthesis by internal adenylation domains of a multidomain enzyme involved in nonribosomal peptide synthesis
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- Abe Tomoko
- Division of Life Science, School of Science and Engineering, Tokyo Denki University
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- Kobayashi Kenta
- Division of Life Science, School of Science and Engineering, Tokyo Denki University
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- Kawamura Sho
- Division of Life Science, School of Science and Engineering, Tokyo Denki University
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- Sakaguchi Tatsuya
- Division of Life Science, School of Science and Engineering, Tokyo Denki University
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- Shiiba Kiwamu
- Division of Life Science, School of Science and Engineering, Tokyo Denki University
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- Kobayashi Michihiko
- Institute of Applied Biochemistry, and Graduate School of Life and Environmental Sciences, The University of Tsukuba
Description
<p>The adenylation domain of nonribosomal peptide synthetase (NRPS) is responsible for its selective substrate recognition and activation of the substrate (yielding an acyl-O-AMP intermediate) on ATP consumption. DhbF is an NRPS involved in bacillibactin synthesis and consists of multiple domains [adenylation domain, condensation domain, peptidyl carrier protein (PCP) domain, and thioesterase domain]; DhbFA1 and DhbFA2 (here named) are "internal" adenylation domains in the multidomain enzyme DhbF. We firstly succeeded in expressing and purifying the "internal" adenylation domains DhbFA1 and DhbFA2 separately. Furthermore, we initially demonstrated dipeptide synthesis by "internal" adenylation domains. When glycine and L-cysteine were used as substrates of DhbFA1, the formation of N-glycyl-L-cysteine (Gly-Cys) was observed. Furthermore, when L-threonine and L-cysteine were used as substrates of DhbFA2, N-L-threonyl-L-cysteine (Thr-Cys) was formed. These findings showed that both adenylation domains produced dipeptides by forming a carbon-nitrogen bond comprising the carboxyl group of an amino acid and the amino group of L-cysteine, although these adenylation domains are acid-thiol ligase using 4'-phosphopantetheine (bound to the PCP domain) as a substrate. Furthermore, DhbFA1 and DhbFA2 synthesized oligopeptides as well as dipeptides.</p>
Journal
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- The Journal of General and Applied Microbiology
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The Journal of General and Applied Microbiology 65 (1), 1-10, 2019
Applied Microbiology, Molecular and Cellular Biosciences Research Foundation