Development of a Three-Dimensional HPLC System for the Simultaneous Determination of Lactate and 3-Hydroxybutyrate Enantiomers in Mammalian Urine
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- HSIEH Chin-Ling
- Graduate School of Pharmaceutical Sciences, Kyushu University School of Pharmacy, Taipei Medical University
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- LIN Po-Yeh
- School of Pharmacy, Taipei Medical University
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- AKITA Takeyuki
- Graduate School of Pharmaceutical Sciences, Kyushu University
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- MITA Masashi
- KAGAMI LAB., Shiseido Global Innovation Center
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- IDE Tomomi
- Graduate School of Medical Sciences, Kyushu University
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- LEE Jen-Ai
- School of Pharmacy, Taipei Medical University
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- HAMASE Kenji
- Graduate School of Pharmaceutical Sciences, Kyushu University School of Pharmacy, Taipei Medical University
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<p>For the highly chemoselective separations of lactate (LA) and 3-hydroxybutyrate (3HB) enantiomers in mammalian urine, an online three-dimensional HPLC system (3D-HPLC) composed of reversed-phase (KSAARP, 1.0 mm i.d. x 250 mm), mixed-mode (KSAAMX, 1.5 mm i.d. x 250 mm) and enantioselective (Chiralpak AD-H, 2.0 mm i.d. x 250 mm) columns has been developed. Following the pre-column derivatization with 4-nitro-7-piperazino-2,1,3-benzoxadiazole (NBD-PZ), NBD-LA and 3HB were separated in the first and second dimensions as their scalmic D plus L mixtures and the enantiomer separations were performed in the third dimension with high separation factors (α = 1.70 for LA; α = 1.43 for 3HB). The present system was applied to the urine of mice and humans. For the mice urine, the D and L-forms of NBD-LA and 3HB were well separated without visible interfering compounds, and trace levels of D-LA (less than 5% of total LA) and non-negligible amounts of L-3HB (34.4% of the total 3HB) were clearly observed. Trace levels of the LA and 3HB enantiomers were detected for the human urine samples, although the existence of a few interfering peaks were observed.</p><p></p>
収録刊行物
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- CHROMATOGRAPHY
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CHROMATOGRAPHY 40 (1), 25-32, 2019-02-20
クロマトグラフィー科学会
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詳細情報 詳細情報について
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- CRID
- 1390564238081479296
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- NII論文ID
- 130007612989
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- NII書誌ID
- AA1137755X
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- ISSN
- 13483315
- 13428284
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- NDL書誌ID
- 029552514
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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