Genetic and Physiological Characteristics of a Novel Marine Propylene-Assimilating <i>Halieaceae</i> Bacterium Isolated from Seawater and the Diversity of Its Alkene and Epoxide Metabolism Genes

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  • Suzuki Toshihiro
    Department of Fermentation Sciences, Faculty of Applied Biosciences, Tokyo University of Agriculture
  • Yazawa Tomoki
    College of Systems Engineering and Science, Shibaura Institute of Technology
  • Morishita Naonori
    College of Systems Engineering and Science, Shibaura Institute of Technology
  • Maruyama Akihiko
    Microbial and Genetic Resources Research Group, Bioproduction Research Institute of Advanced Industrial Science and Technology (AIST)
  • Fuse Hiroyuki
    College of Systems Engineering and Science, Shibaura Institute of Technology

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Other Title
  • Genetic and Physiological Characteristics of a Novel Marine Propylene-Assimilating Halieaceae Bacterium Isolated from Seawater and the Diversity of Its Alkene and Epoxide Metabolism Genes

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Abstract

<p>The Gram-negative marine propylene-assimilating bacterium, strain PE-TB08W, was isolated from surface seawater. A structural gene analysis using the 16S rRNA gene showed 96, 94, and 95% similarities to Halioglobus species, Haliea sp. ETY-M, and Haliea sp. ETY-NAG, respectively. A phylogenetic tree analysis showed that strain PE-TB08W belonged to the EG19 (Chromatocurvus)-Congregibacter-Haliea cluster within the Halieaceae (formerly Alteromonadaceae) family. Thus, strain PE-TB08W was characterized as a newly isolated Halieaceae bacterium; we suggest that this strain belongs to a new genus. Other bacterial characteristics were investigated and revealed that strain PE-TB08W assimilated propylene, n-butane, 1-butene, propanol, and 1-butanol (C3 and C4 gaseous hydrocarbons and primary alcohols), but not various other alcohols, including methane, ethane, ethylene, propane, and i-butane. The putative alkene monooxygenase (amo) gene in this strain was a soluble methane monooxygenase-type (sMMO) gene that is ubiquitous in alkene-assimilating bacteria for the initial oxidation of alkenes. In addition, two epoxide carboxylase systems containing epoxyalkane, the co-enzyme M transferase (EaCoMT) gene, and the co-enzyme M biosynthesis gene, were found in the upstream region of the sMMO gene cluster. Both of these genes were similar to those in Xanthobacter autotrophicus Py2 and were inductively expressed by propylene. These results have a significant impact on the genetic relationship between terrestrial and marine alkene-assimilating bacteria.</p>

Journal

  • Microbes and Environments

    Microbes and Environments 34 (1), 33-42, 2019

    Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles

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