Development of a sandwich ELISA for the carboxylesterase 5A fragment detected by serum proteome analysis.

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  • サンドイッチELISA法を用いたCarboxylesterase 5A fragment測定系の構築と評価
  • サンドイッチ ELISAホウ オ モチイタ Carboxylesterase 5A fragment ソクテイケイ ノ コウチク ト ヒョウカ

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Chronic kidney disease (CKD) is a common disorder and cause of death in cats. We previously identified novel biomarker candidates in CKD using urine proteome analysis. We developed an enzyme immunoassay that enables the measurement of urine carboxylesterase 5A fragment levels. The ELISA performance was satisfactory in terms of recovery (96.1-106.4%), and within-run (3.5-5.1%) and between-day (4.8-8.2%) reproducibility. The sandwich ELISA developed in this study will be useful to confirm the diagnostic significance of urine carboxylesterase 5A fragment levels.

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