Practical Techniques of Cryo-electron Tomography

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  • Oda Toshiyuki
    山梨大学大学院総合研究部医学域解剖学講座構造生物学教室

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Other Title
  • クライオ電子トモグラフィーの実用技術
  • クライオ デンシ トモグラフィー ノ ジツヨウ ギジュツ

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Abstract

<p>Cryo-electron tomography is a powerful method to visualize the three-dimensional structures of macromolecular complexes and cellular organelles in situ. Compared with the single particle analysis, however, cryo-electron tomography appears to be relatively unpopular probably due to the seemingly complicated methodology. In this review, I will give an overview of the practical aspects of the cryo-electron tomography as well as the recent progress in this field of research.</p>

Journal

  • KENBIKYO

    KENBIKYO 53 (1), 18-23, 2018-04-30

    The Japanese Society of Microscopy

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